Effect of perfluorooctane sulfonate on pluripotency and differentiation factors in mouse embryoid bodies. (3rd February 2015)

• Record Type: Journal Article
• Authors: Xu, Bo; Ji, Xiaoli; Chen, Xiaojiao; Yao, Mengmeng; Han, Xiumei; Chen, Minjian; Tang, Wei; Xia, Yankai
• Is Part Of: Toxicology. Volume 328(2015)
• Page Start: 160
• Page End: 167
• Publication Date: 2015-02-03
• Subjects: PFOS perfluorooctane sulfonate -- mEBs mouse embryoid bodies -- PcG polycomb group -- MiR microRNA; Perfluorooctane sulfonate -- Mouse embryoid bodies -- Pluripotency -- Differentiation -- MicroRNA -- Polycomb group; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2014.12.010
• Languages: English
• ISSNs: 0300-483X

Abstract

Abstract: Perfluorooctane sulfonate (PFOS) poses potential risks to early development, but the molecular mechanisms how PFOS affects embryonic development are still unclear. Mouse embryoid bodies (mEBs) provide ideal models for testing safety or toxicity of chemicals in vitro . In this study, mEBs were exposed to PFOS up to 6 days and then their pluripotency and differentiation markers were evaluated. Our data showed that the mRNA and protein levels of pluripotency markers (Oct4, Sox2, Nanog) in mEBs were significantly increased following exposure to PFOS. Meanwhile, the expressions of miR - 134, miR - 145, miR - 490 - 3p were decreased accordingly. PFOS reduced the mRNA levels of endodermal markers (Sox17, FOXA2), mesodermal markers (SMA, Brachyury) and ectodermal markers (Nestin, Fgf5) in mEBs. Meanwhile, PFOS increased the mRNA and protein levels of polycomb group (PcG) family members (Cbx4, Cbx7, Ezh2). Overall, our results showed that PFOS could increase the expression levels of pluripotency factors and decrease the differentiation markers.