Bioaffinity limitations for anisotropically etched silicon microfluidics. (26th April 2010)

• Record Type: Journal Article
• Authors: Alazzam, Anas; Chandrasekaran, Arvind; Packirisamy, Muthukumaran; Stiharu, Ion; , Rama Bhat
• Is Part Of: International journal of abrasive technology. Volume 3:Number 2(2010)
• Page Start: 122
• Page End: 132
• Publication Date: 2010-04-26
• Subjects: micro total analysis systems -- &amp -- mu -- TASs -- lab-on-a-chip -- biosensing -- microfluidics -- finite element analysis -- FEA -- immobilisation -- anisotropic etching -- silicon etching -- bioaffinity -- stagnant flow -- biomicrofluidics -- Navier-Stokes equation; Grinding and polishing -- Periodicals; Abrasives -- Periodicals; 620.44
• Languages: English
• ISSNs: 1752-2641

Abstract

Immobilisation of biological specimens is an essential process in biomicrofluidics for micro total analysis systems (μTASs) applications, to carry out high throughput bioassays. Closed microfluidic channels restrict immobilisation feasibilities to only direct conjugation of the biomolecules with the channel surface through appropriate flow control-based incubation techniques. In this work, the limitations of bioaffinity on anisotropically etched silicon microfluidic channels have been studied. The flow behaviour within the microchannels has been analysed by solving the Navier-Stokes equation using finite element method (FEM). The results have been used to predict the immobilisation and rinsing behaviour within anisotropically etched trapezoidal and triangular microfluidic channels.