A Ca-alginate particle co-immobilized with Phanerochaete chrysosporium cells and the combined cross-linked enzyme aggregates from Trametes versicolor. (December 2015)

• Record Type: Journal Article
• Authors: Li, Yanchun; Wang, Zhi; Xu, Xudong; Jin, Liqiang
• Is Part Of: Bioresource technology. Volume 198(2015)
• Page Start: 464
• Page End: 469
• Publication Date: 2015-12
• Subjects: Combi-CLEAs -- Co-immobilization -- White-rot fungi -- Dye -- Heavy metal ionic; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.09.032
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The combi-CLEAs of ligninolytic enzymes from Trametes versicolor was obtained. The Phanerochaete chrysosporium cells and the combi-CLEAs were co-immobilized. The particles have great decolorizing ability and heavy metal ionic resistibility. Abstract: For improving stability of immobilized white-rot fungus to treat various effluents, Phanerochaete chrysosporium cells and the combined cross-link enzyme aggregates (combi-CLEAs) prepared from Trametes versicolor were co-immobilized into the Ca-alginate gel particles in this paper. The activity yields of obtained combi-CLEAs were 42.7% for lignin peroxidases (LiPs), 31.4% for manganese peroxidases (MnPs) and 40.4% for laccase (Lac), respectively. And their specific activities were 30.2 U/g as combi-CLEAs-LiPs, 9.5 U/g as combi-CLEAs-MnPs and 28.4 U/g as combi-CLEAs-Lac. Further, the present of the combi-CLEAs in the particles extremely improved their ability to degrade the dyes. Compared to the immobilized Ph. chrysosporium without the combi-CLEAs, the co-immobilized particles enhanced the decolorized rate of Acid Violet 7 (from 45.2% to 93.4%) and Basic Fuchsin (from 12.1% to 67.9%). In addition, the addition of the combi-CLEAs improved the adaptability of the white-rot fungal particles to adverse environmental conditions.