Identification of HSP70-inducing activity in Arnica montana extract and purification and characterization of HSP70-inducers. Issue 1 (April 2015)

• Record Type: Journal Article
• Authors: Usui, Keiko; Ikeda, Tsuyoshi; Horibe, Yuumi; Nakao, Mitsuyoshi; Hoshino, Tatsuya; Mizushima, Tohru
• Is Part Of: Journal of dermatological science. Volume 78:Issue 1(2015:Apr.)
• Page Start: 67
• Page End: 75
• Publication Date: 2015-04
• Subjects: A. montana Arnica montana -- DMEM Dulbecco's modified Eagle's medium -- E. lindleyanum Eupatorium lindleyanum -- FBS fetal bovine serum -- GGA geranylgeranylacetone -- HPLC high performance liquid chromatography -- HSF-1 heat shock factor-1 -- HSP heat shock protein -- IBMX 3-isobutyl-1-methylxanthine -- MS mass spectra -- MTT 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- NF-κB nuclear factor kappa B -- NMR nuclear magnetic resonance -- ODS octadecylsilane -- PBS phosphate buffered saline -- S.D. standard deviation -- UV ultraviolet; Heat shock protein 70 -- Arnica montana -- Photo-aging -- Melanogenesis -- Hypopigmenting cosmetics; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2015.01.014
• Languages: English
• ISSNs: 0923-1811

Abstract

Highlights: We here searched for HSP70-inducers from a library of herbal extracts that have already been approved as quasi-pharmaceutical products in Japan. We selected an ethanol extract of Arnica montana and purified an HSP70-inducer, AM-2 (helenalin 2-methylbutyrate). Pre-treatment of cells with AM-2 or Arnica montana extract decreased melanin production. These results suggest that AM-2 and Arnica montana extract could be beneficial for use in hypopigmenting cosmetics as a consequence of their stimulatory effects on HSP70 expression. Abstract: Background: The expression of heat shock proteins (HSPs), particularly HSP70, is receiving considerable attention in the field of cosmetics, particularly given our recent report that ultraviolet-induced melanin production, skin damage and wrinkle formation were all suppressed in transgenic mice expressing HSP70. Objective: In the present study, we searched for HSP70-inducers from a library of herbal extracts that have already been approved as quasi-pharmaceutical products in Japan. We selected an ethanol extract of Arnica montana ( A. montana ), based on its high HSP70-inducing activity and low cytotoxicity. Methods: Cell viability was determined by MTT method and expression of HPS70 was monitored by immunoblotting analysis. Results: From the extract, we purified and identified eight sesquiterpene lactones (AM1–8) as HSP70-inducers, among which AM-2 (helenalin 2-methylbutyrate) was selected due to its good HSP70-inducing properties and low cytotoxicity. Treatment of cultured mouse melanoma cells with AM-2 or A. montana extract up-regulated the expression of HSP70 in a dose-dependent manner. This treatment also activated heat shock factor-1, a transcription factor for hsp genes. Furthermore, pre-treatment of cells with AM-2 or A. montana extract decreased melanin production and expression and activity of tyrosinase. Conclusion: These results suggest that AM-2 and A. montana extract could be beneficial for use in hypopigmenting cosmetics as a consequence of their stimulatory effects on HSP70 expression.