Conceptual and technical aspects of transfection and gene delivery. Issue 6 (15th March 2015)

• Record Type: Journal Article
• Authors: Kaestner, Lars; Scholz, Anke; Lipp, Peter
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 25:Issue 6(2015)
• Page Start: 1171
• Page End: 1176
• Publication Date: 2015-03-15
• Subjects: Gene transfer -- Gene therapy -- Transduction -- Lipofection -- Electroporation; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2015.01.018
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Genetically modified animals are state of the art in biomedical research as gene therapy is a promising perspective in the attempt to cure hereditary diseases. Both approaches have in common that modified or corrected genetic information must be transferred into cells in general or into particular cell types of an organism. Here we give an overview of established and emerging methods of transfection and gene delivery and provide conceptual and technical advantages and drawbacks of their particular use. Additionally, based on a flow chart, we compiled a rough guideline to choose a gene transfer method for a particular field of application.