A new method to rapidly detect rusty grain beetle, Cryptolestes ferrugineus (Stephens), in stored grain. (July 2015) - Jian, Fuji
Abstract: Information on insect infestation inside stored grain bulks is required for safe grain storage. A new method to rapidly detect both adults and larvae of Cryptolestes ferrugineus (Stephens) in grain was developed based on the principle of microwave heating and insect behaviour under elevated temperature. The designed apparatus and processing procedure were tested to extract both the adults and larvae inside wheat with 14%, 16% and 18% moisture contents by using a domestic microwave oven (referred to as the microwave method). The recovery percentage of the introduced insects associated with the microwave method was compared with that of the Berlese funnel method (810 cm 3 wheat in a funnel under an incandescent light bulb). The microwave method recovered 97.8% of introduced adults, while 90.6% of adults were recovered by the Berlese funnel method. The recovery percentage of the larvae inside marked wheat kernels was 83.3 ± 3.3% with the microwave method and less than 27% with the Berlese funnel method. There was no significant difference in extraction percentage between old and young larvae when the microwave method was used. The moisture content of the treated grain did not significantly influence the extraction percentage of the pest insect. The total processing time of the microwave method was less than 30 min compared to 6 h required for the Berlese funnel method. Highlights: A new method to rapidly detect both adults and larvae was developed. The new method extracted more insects than the Berlese funnel method. Moisture content of the treated grain did not influence the extraction percentage. The new method could extract insects in less than 30 min.