Descriptive assessment of uncertainties of qualitative real‐time PCR for detection of plant pathogens and quality performance monitoring. (17th December 2014)

• Record Type: Journal Article
• Authors: Mehle, N.; Dreo, T.; Jeffries, C.; Ravnikar, M.
• Is Part Of: Bulletin OEPP. Volume 44:Number 3(2014)
• Page Start: 502
• Page End: 509
• Publication Date: 2014-12-17
• Subjects: Plant diseases -- Periodicals; Plants, Protection of -- Periodicals
• DOI: 10.1111/epp.12166
• Languages: English
• ISSNs: 0250-8052

Abstract

Abstract : In most cases, plant pest diagnostic methods are interpreted as providing qualitative data. However, although qualitative, it is still important to identify all the sources and components of uncertainty in the diagnostic process and to ensure that appropriate measures are taken to reduce the uncertainty and that the form of reporting the result does not give the wrong impression of the uncertainty. This process was illustrated using real‐time PCR testing of grapevine yellows phytoplasma. Some additional quality performance monitoring of real‐time PCR is also described. This approach has been used by the National Institute of Biology, Slovenia, since 2012, in support of accreditation under ISO 17025, and it can also be applied to other detection methods and other pests.