Single cell analysis using a glass microchamber for studying movement fluctuations of Navicula pavillardii and Seminavis robusta diatom cells. (October 2015)

• Record Type: Journal Article
• Authors: Umemura, Kazuo; Sadoya, Yusuke; Nagao, Kazuki; Oikawa, Ryota; Hanada, Yasutaka; Sugioka, Koji; Mayama, Shigeki
• Is Part Of: Micron. Volume 77(2015:Oct.)
• Page Start: 41
• Page End: 43
• Publication Date: 2015-10
• Subjects: Diatom -- Microchamber -- Trajectory -- Gliding -- Angular velocity; Microscopy -- Periodicals; Electron Probe Microanalysis -- Periodicals; Microscopy -- Periodicals; Microscopie -- Périodiques; Microscopy; Periodicals; 502.82
• DOI: 10.1016/j.micron.2015.05.005
• Languages: English
• ISSNs: 0968-4328

Abstract

Highlights: A glass microchamber was effective on the trajectory analysis of diatom cells. Angular velocities of the cell movements were calculated by trajectory analysis. Fluctuation of the angular velocity was quantitatively analyzed. SR showed higher fluctuation of the angular velocity. Abstract: We propose a new technique for quantitative trajectory analysis of gliding phenomenon of Navicula pavillardii (NP) and Seminavis robusta (SR) diatom cells by single cell observation using a glass microchamber in this short technical note. Two-dimensional trajectory analysis of cell movements was used to determine the angular velocity, velocity, and migration distances of the diatom movement. Based on the trajectory analysis, we found that asymmetrically shaped SR had a larger angular velocity with large fluctuations compared to symmetrically shaped NP, although the velocity of SR was less than that of NP. It suggests that lateral frictional force in a culture medium is an important factor for diatom movements. Our results revealed that the single cell observation using a glass microchamber is effective on quantitative analysis of angular velocity of diatom gliding.