Optimization of EGFR high positive cell isolation procedure by design of experiments methodology. Issue 5 (18th April 2015)

• Record Type: Journal Article
• Authors: Levi, Ofer; Tal, Baruch; Hileli, Sagi; Shapira, Assaf; Benhar, Itai; Grabov, Pavel; Eliaz, Noam
• Is Part Of: Cytometry. Volume 88:Issue 5(2015)
• Page Start: 338
• Page End: 347
• Publication Date: 2015-04-18
• Subjects: bio‐ferrography -- carcinoma -- design of experiments -- EGFR -- flow cytometry -- immunomagnetic isolation; Flow cytometry -- Diagnostic use -- Periodicals; Cytodiagnosis -- Periodicals; 616.07582
• DOI: 10.1002/cyto.b.21246
• Languages: English
• ISSNs: 1552-4949

Abstract

Abstract : Background: Circulating tumor cells (CTCs) in blood circulation may play a role in monitoring and even in early detection of metastasis patients. Due to the limited presence of CTCs in blood circulation, viable CTCs isolation technology must supply a very high recovery rate. Methods: Here, we implement design of experiments (DOE) methodology in order to optimize the Bio‐Ferrography (BF) immunomagnetic isolation (IMI) procedure for the EGFR high positive CTCs application. All consequent DOE phases such as screening design, optimization experiments and validation experiments were used. Results: A significant recovery rate of more than 95% was achieved while isolating 100 EGFR high positive CTCs from 1 mL human whole blood. Conclusions: The recovery achievement in this research positions BF technology as one of the most efficient IMI technologies, which is ready to be challenged with patients' blood samples. © 2015 International Clinical Cytometry Society