Smart nanocarrier based on PEGylated hyaluronic acid for deacetyl mycoepoxydience: High stability with enhanced bioavailability and efficiency. (1st January 2019)

• Record Type: Journal Article
• Authors: Zhao, Juan; Wang, Yancai; Ma, Yingying; Liu, Yangyang; Yan, Beibei; Wang, Lulu
• Is Part Of: Carbohydrate polymers. Volume 203(2018)
• Page Start: 356
• Page End: 368
• Publication Date: 2019-01-01
• Subjects: Pyridine (PubChem CID: 1049) -- 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide (PubChem CID: 15908) -- N-hydroxysuccinimide (PubChem CID: 80170) -- Polyethylene glycol (PubChem CID: 174 (Ethylene glycol)) -- Folic acid (PubChem CID: 6037) -- Hyaluronate sodium (PubChem CID: 3084049) -- Vitamin E TPGS (PubChem CID: 71406) -- Ethanol (PubChem CID: 702) -- Distearoyl phosphatidyl ethanolamine (PubChem CID: 102547) -- Dimethyl sulfoxide (PubChem CID: 679); Nanocrystals -- Deacetyl mycoepoxydience -- Core-shelled structured -- PEGylated -- Hyaluronic acid -- Pharmacokinetics; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.09.071
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A core-shelled structure drug delivery system was designed. The DM nanocrystals core was prepared by anti-solvent precipitation method. PEG-HA shell possesses pH-responsible ability of release drug in suitable position. TPGS and DSPE-PEG-FA as functional stabilizers for stable nanocrystals. Response surface methodology for select optimum DM nanosuspensions formulation. Abstract: Deacetyl mycoepoxydience (DM) nanocrystals core were stabilized by the folate modified distearoylphosphatidyl ethanolamine-polyethylene glycol (DSPE-PEG2000 -FA) as the active-targeting stabilizer and D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) as the reversion of multidrug resistance stabilizer, respectively. The DM nanocrystals was acted as the core and shelled by the polyethylene glycol-hyaluronic acid (PEG-HA). The optimal core-shell system demonstrated superior stability at 4 °C for 6 weeks by the stability study and higher dissolution velocity. Cytotoxicity in vitro and cell proliferation inhibition was evaluated by MCF-7 cells line. Furthermore, the core-shell nanocrystals revealed a concentration- and time-dependent cytotoxicity activity and enhanced the cell proliferation inhibition. Pharmacokinetic studies in rabbits showed core-shelled DM nanocrystals significantly increased AUC and t1/2 and reduced CLz compared to the DM solution for intravenous delivery. Results indicated that core-shell nanocrystals nanogel was successfully established with higher stability and the bioavailability of DM with higher safety was improved.