Deep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1‐mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean. (10th June 2016)

• Record Type: Journal Article
• Authors: Chen, Hui; Adam Arsovski, Andrej; Yu, Kangfu; Wang, Aiming
• Is Part Of: Molecular plant pathology. Volume 18:Number 3(2017)
• Page Start: 391
• Page End: 404
• Publication Date: 2016-06-10
• Subjects: degradome‐seq -- eukaryotic translation initiation factor -- lethal systemic hypersensitive response -- Soybean mosaic virus -- sRNA‐seq -- transcriptome‐seq -- virus–host interaction; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12407
• Languages: English
• ISSNs: 1464-6722

Abstract

Summary: Rsv1, a single dominant resistance locus in soybean, confers extreme resistance to the majority of Soybean mosaic virus (SMV) strains, but is susceptible to the G7 strain. In Rsv1 ‐genotype soybean, G7 infection provokes a lethal systemic hypersensitive response (LSHR), a delayed host defence response. The Rsv1 ‐mediated LSHR signalling pathway remains largely unknown. In this study, we employed a genome‐wide investigation to gain an insight into the molecular interplay between SMV G7 and Rsv1 ‐genotype soybean. Small RNA (sRNA), degradome and transcriptome sequencing analyses were used to identify differentially expressed genes (DEGs) and microRNAs (DEMs) in response to G7 infection. A number of DEGs, DEMs and microRNA targets, and the interaction network of DEMs and their target mRNAs responsive to G7 infection, were identified. Knock‐down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7‐induced, Rsv1 ‐mediated LSHR signalling pathway. This work provides an in‐depth genome‐wide analysis of high‐throughput sequencing data, and identifies multiple genes and microRNA signatures that are associated with the Rsv1 ‐mediated LSHR.