Comparison of haemostatic function of PAS‐C–platelets vs. plasma–platelets in reconstituted whole blood using impedance aggregometry and thromboelastography. Issue 6 (9th June 2017)

• Record Type: Journal Article
• Authors: van Hout, F. M. A.; Bontekoe, I. J.; de Laleijne, L. A. E.; Kerkhoffs, J.‐L.; de Korte, D.; Eikenboom, J.; van der Bom, J. G.; van der Meer, P. F.
• Is Part Of: Vox sanguinis. Volume 112:Issue 6(2017)
• Page Start: 549
• Page End: 556
• Publication Date: 2017-06-09
• Subjects: CD62P expression -- haemostatic function -- multiple electrode aggregometry -- platelet additive solution -- thromboelastography; Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/vox.12534
• Languages: English
• ISSNs: 0042-9007

Abstract

Abstract : Background and Objectives: There are concerns about the haemostatic function of platelets stored in platelet additive solution (PAS). Aim of this study was to compare the haemostatic function of PAS‐C–platelets to plasma–platelets in reconstituted whole blood. Materials and Methods: In our experiment, whole blood was reconstituted with red blood cells, solvent–detergent (SD) plasma and either PAS‐C–platelets or plasma–platelets ( n = 7) in a physiological ratio. On storage days 2, 5, 8 and 13, the agonist‐induced aggregation (multiple electrode aggregometry), clot formation (thromboelastography) and agonist‐induced CD62P responsiveness (flow cytometry) were measured. Results: Samples with PAS‐C–platelets showed significantly lower aggregation than plasma–platelets when induced with adenosine diphosphate, −6 U (95% confidence interval: −8; −4) or thrombin receptor‐activating protein, −15 U (−19; −10). Also when activated with collagen and ristocetin, the PAS‐C–platelets showed less aggregation, although not statistically significant. All samples with PAS‐C–platelets showed significantly lower agonist‐induced CD62P responsiveness than samples with plasma–platelets. However, there was no difference regarding all TEG parameters. Conclusion: Our findings demonstrate that the function – aggregation and CD62P responsiveness – of PAS‐C–platelets in reconstituted whole blood is inferior to that of plasma–platelets, which may have implications in the setting of massive transfusions.