Characterization of a high field Orbitrap mass spectrometer for proteome analysis. Issue 17 (30th July 2013)

• Record Type: Journal Article
• Authors: Pachl, Fiona; Ruprecht, Benjamin; Lemeer, Simone; Kuster, Bernhard
• Is Part Of: Proteomics. Volume 13:Issue 17(2013:Sep.)
• Page Start: 2552
• Page End: 2562
• Publication Date: 2013-07-30
• Subjects: High field Orbitrap -- LC‐MS/MS -- Orbitrap Elite -- Protein identification -- Technology; Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201300076
• Languages: English
• ISSNs: 1615-9853

Abstract

Abstract : The field of proteomics continues to be driven by improvements in analytical technology, notably in peptide separation, quantitative MS, and informatics. In this study, we have characterized a hybrid linear ion trap high field Orbitrap mass spectrometer (Orbitrap Elite) for proteomic applications. The very high resolution available on this instrument allows 95% of all peptide masses to be measured with sub‐ppm accuracy that in turn improves protein identification by database searching. We further confirm again that mass accuracy in tandem mass spectra is a valuable parameter for improving the success of protein identification. The new CID rapid scan type of the Orbitrap Elite achieves similar performance as higher energy collision induced dissociation fragmentation and both allow the identification of hundreds of proteins from as little as 0.1 ng of protein digest on column. The new instrument outperforms its predecessor the Orbitrap Velos by a considerable margin on each metric assessed that makes it a valuable and versatile tool for MS‐based proteomics.