Acute kidney damage induced by low‐ and iso‐osmolar contrast media in rats: Comparison study with physiologic MRI and histologic‐gene examination. Issue 1 (1st July 2016)

• Record Type: Journal Article
• Authors: Wu, Chen‐Jiang; Bao, Mei‐Ling; Wang, Qing; Wang, Xiao‐Ning; Liu, Xi‐Sheng; Shi, Hai‐Bin; Zhang, Yu‐Dong
• Is Part Of: Journal of magnetic resonance imaging. Volume 45:Issue 1(2017)
• Page Start: 291
• Page End: 302
• Publication Date: 2016-07-01
• Subjects: contrast‐induced acute kidney injury -- diffusion‐weighted imaging -- dynamic MR renography -- renal function; Magnetic resonance imaging -- Periodicals; 616
• DOI: 10.1002/jmri.25346
• Languages: English
• ISSNs: 1053-1807

Abstract

Abstract : Purpose: To investigate the physiopathological effects of low‐ and iso‐osmolar contrast media (CM) on renal function with physiologic MRI and histologic‐gene examination. Materials and Methods: Forty‐eight rats underwent time‐course DWI and DCE‐MRI at 3.0 Tesla (T) before and 5–15 min after exposure of CM or saline (Iop.370: 370 mgI/mL iopromide; Iod.320: 320 mgI/mL iodixanol; Iod.270: 270 mgI/mL iodixanol; 4 gI/kg body weight). Intrarenal viscosity was reflected by apparent diffusion coefficient (ADC). Renal physiologies were evaluated by DCE‐derived glomerular filtration rate (GFR), renal blood flow (RBF), and renal blood volume (RBV). Potential acute kidney injury (AKI) was determined by histology and the expression of kidney injury molecule 1 (Kim‐1). Results: Iop.370 mainly increased ADC in inner‐medulla (△ADCIM : 12.3 ± 11.1%; P < 0.001). Iod.320 and Iod.270 mainly decreased ADC in outer‐medulla (△ADCIM ; Iod.320: 16.8 ± 7.5%; Iod.270: 18.1 ± 9.5%; P < 0.001) and inner‐medulla (△ADCIM ; Iod.320: 28.4 ± 9.3%; Iod.270: 30.3 ± 6.3%; P < 0.001). GFR, RBF and RBV were significantly decreased by Iod.320 (△GFR: 45.5 ± 24.1%; △RBF: 44.6 ± 19.0%; △RBV: 35.2 ± 10.1%; P < 0.001) and Iod.270 (33.2 ± 19.0%; 38.1 ± 15.6%; 30.1 ± 10.1%; P < 0.001), while rarely changed by Iop.370 and saline. Formation of vacuoles and increase in Kim‐1 expression was prominently detected in group of Iod.320, while rarely in Iod.270 and Iop.370. Conclusion: Iso‐osmolar iodixanol, given at high‐dose, produced prominent AKI in nonhydrated rats. This renal dysfunction could be assessed noninvasively by physiologic MRI. Level of Evidence: 1 J. Magn. Reson. Imaging 2017;45:291–302.