Hematopoietic progenitor cells collection in pediatric patients with brain tumor. Issue 1 (7th April 2015)

• Record Type: Journal Article
• Authors: Spínola, Ana; Ferreira, Sara; Amado, Fátima; Lopes, Sérgio; Roncon, Susana
• Is Part Of: Journal of clinical apheresis. Volume 31:Issue 1(2016)
• Page Start: 22
• Page End: 28
• Publication Date: 2015-04-07
• Subjects: hematopoietic stem cells -- leukapheresis -- brain tumor -- pediatric patients; Hemapheresis -- Periodicals; Blood -- Transfusion -- Periodicals; Blood -- Transfusion, Autologous -- Periodicals; Cell separation -- Periodicals; Leukapheresis -- Periodicals; Plasmapheresis -- Periodicals; 615.39
• DOI: 10.1002/jca.21398
• Languages: English
• ISSNs: 0733-2459

Abstract

Abstract : Aim: To analyze the efficacy and safety of hematopoietic progenitor cells (HPC) collections by leukapheresis in pediatric patients with brain tumors. Results: Between 2003 and 2014, we collected HPC from 19 children (12 boys/7 girls), median age at the diagnosis of 5 years old (<1–15 years old) and weight of 16.8 Kg (6.7–42). Diagnoses were Medulloblastoma ( n = 10), Primitive Neuroectodermal Tumor ( n = 5), Atypical Teratoid Rhabdoid Tumor ( n = 3) and Secreting Germ Cell Tumor ( n = 1). All patients performed leukapheresis by a central venous catheter, at the fifth day of mobilization with G‐CSF (median dose 11.7 µg/Kg/day), 9 of them with COBE® Spectra and 10 with Spectra Optia®. The anticoagulant used was ACD‐A, ratio of 14:1 or ACD‐A plus heparin, ratio 25:1. The tubing set was primed with a compatible, irradiated, leukodepleted and hematocrit adjusted packed red cells for all children <30 kg ( n = 17). A median of 1.5 (1–3) leukapheresis per patient was performed with an average of 3 (1.5–5.4) blood volumes processed; 3 children did a second mobilization and one additional leukapheresis. The median number of CD34+ cells collected was 4.6 × 10 6 /Kg (0.18–22.6) of patient body weight; 12 children collected for a tandem transplant. The median time between cell collection and infusion was 3 (0.6–9.1) months. Conclusions: HPC collection in children is an efficient and well tolerated technique, performed as an outpatient procedure. With the new mobilization schemes and leukapheresis technology, we can collect a high number of HPC allowing pediatric oncologist to establish more aggressive chemotherapy protocols hoping to improve patient outcome. J. Clin. Apheresis 31:22–28, 2016. © 2015 Wiley Periodicals, Inc.