Mesoderm Lineage 3D Tissue Constructs Are Produced at Large‐Scale in a 3D Stem Cell Bioprocess. Issue 9 (9th August 2017)

• Record Type: Journal Article
• Authors: Cha, Jae Min; Mantalaris, Athanasios; Jung, Sunyoung; Ji, Yurim; Bang, Oh Young; Bae, Hojae
• Is Part Of: Biotechnology journal. Volume 12:Issue 9(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-08-09
• Subjects: 3D stem cell bioprocess -- biomolecular microenvironment -- mesoderm lineage tissue -- large scale production; Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.201600748
• Languages: English
• ISSNs: 1860-6768

Abstract

Abstract : Various studies have presented different approaches to direct pluripotent stem cell differentiation such as applying defined sets of exogenous biochemical signals and genetic/epigenetic modifications. Although differentiation to target lineages can be successfully regulated, such conventional methods are often complicated, laborious, and not cost‐effective to be employed to the large‐scale production of 3D stem cell‐based tissue constructs. A 3D‐culture platform that could realize the large‐scale production of mesoderm lineage tissue constructs from embryonic stem cells (ESCs) is developed. ESCs are cultured using our previously established 3D‐bioprocess platform which is amenable to mass‐production of 3D ESC‐based tissue constructs. Hepatocarcinoma cell line conditioned medium is introduced to the large‐scale 3D culture to provide a specific biomolecular microenvironment to mimic in vivo mesoderm formation process. After 5 days of spontaneous differentiation period, the resulting 3D tissue constructs are composed of multipotent mesodermal progenitor cells verified by gene and molecular expression profiles. Subsequently the optimal time points to trigger terminal differentiation towards cardiomyogenesis or osteogenesis from the mesodermal tissue constructs is found. A simple and affordable 3D ESC‐bioprocess that can reach the scalable production of mesoderm origin tissues with significantly improved correspondent tissue properties is demonstrated. Abstract : Conventional methods for stem cell differentiation are often complicated, laborious, and not cost‐effective to be employed to the large‐scale production of 3D stem cell‐based tissue constructs. The large‐scale production of mesoderm lineage tissue constructs from embryonic stem cells (ESCs) is achieved through the initial treatment of hepatocarcinoma cell line conditioned medium to the 3D‐ESCs encapsulated within alginate/gelatin hydrogel that are cultured using a rotating wall vessel bioreactor. The simple 3D stem cell bioprocess present in this study can contribute to the "off‐the‐shelf" production of mesoderm origin tissues such as cardiac and skeletal tissue constructs.