Reduced Insulin Resistance Contributes to the Beneficial Effect of Protein Tyrosine Phosphatase-1B Deletion in a Mouse Model of Sepsis. Issue 3 (September 2017)

• Record Type: Journal Article
• Authors: Delile, Eugénie; Nevière, Rémi; Thiébaut, Pierre-Alain; Maupoint, Julie; Mulder, Paul; Coquerel, David; Renet, Sylvanie; Rieusset, Jennifer; Richard, Vincent; Tamion, Fabienne
• Is Part Of: Shock. Volume 48:Issue 3(2017)
• Publication Date: 2017-09
• Subjects: Glucose -- hyperglycemia -- insulin resistance -- PTP1B -- severe sepsis -- vascular dysfunction -- Akt -- kinase protein B -- AMPK -- adenosine monophosphate-activated protein kinase -- CD45 -- cluster of differenciation 45 -- CLP -- cecal ligation and puncture -- DNA -- desoxyribo nucleotide acid -- eNOS -- endothelial nitric oxide synthase -- FMD -- flow-mediated dilatation -- GLUT-1 -- glucose transporter 1 -- GLUT-4 -- glucose transporter 4 -- GLUTs -- glucose transporters -- HOMA -- homeostasis model assessment -- ICAM-1 -- intercellular adhesion molecule 1 -- ICU -- intensive care unit -- IL-1β -- interleukin-1β -- IL-10 -- interleukin-10 -- IL-6 -- interleukin-6 -- iNOS -- inducible nitric oxide synthase -- IR -- insulin receptors -- IRS-1 -- insulin receptor substrate 1 -- IRSs -- insulin receptor substrates -- KH -- Krebs–Henseleit -- L-NNA -- Nω-nitro-L-arginine -- LPS -- lipopolysaccharide -- mRNA -- messenger ribonucleic acid -- MVO2 -- myocardial oxygen uptake -- NF-κB -- nuclear factor-kappa B -- NO -- nitric oxide -- PCR -- polymerase chain reaction -- Phe -- phenylephrine -- PI3K -- phospho-inositol-3-kinase -- PTP1B -- protein tyrosine phosphatase 1B -- TLR4 -- toll-like receptor 4 -- TNF-α -- tumor necrosis factor-α -- VCAM1 -- vascular cell adhesion molecule 1 -- WT -- wild type; Shock -- Periodicals; Shock -- Periodicals; Choc (Pathologie) -- Périodiques; Shock; Periodicals; 616.0475
• DOI: 10.1097/SHK.0000000000000853
• Languages: English
• ISSNs: 1073-2322

Abstract

Abstract : ABSTRACT: Hyperglycemia is a common feature of septic patients and has been associated with poor outcome and high mortality. In contrast, insulin has been shown to decrease mortality and to prevent the incidence of multiorgan failure but is often associated with deleterious hypoglycemia. Protein Tyrosine Phosphatase 1B (PTP1B) is a negative regulator of both insulin signaling and NO production, and has been shown to be an aggravating factor in septic shock. To evaluate the potential therapeutic effect of PTP1B blockade on glucose metabolism and insulin resistance in an experimental model of sepsis, we assessed the effect of PTP1B gene deletion in a cecal ligation and puncture (CLP) model of sepsis. PTP1B gene deletion significantly limited CLP-induced insulin resistance, improved AMP-activated protein kinase signaling pathway and Glucose Transporter 4 translocation, and decreased inflammation. These effects were associated with a reduction of sepsis-induced endothelial dysfunction/impaired NO production and especially of insulin-mediated dilatation. This modulation of insulin resistance may contribute to the beneficial effect of PTP1B blockade in septic shock, especially in terms of inflammation and cardiac metabolism. Abstract : Supplemental Digital Content is available in the text