Intratumoral heterogeneity of steroidogenesis in aldosterone-producing adenoma revealed by intensive double- and triple-immunostaining for CYP11B2/B1 and CYP17. (15th February 2016)

• Record Type: Journal Article
• Authors: Nakamura, Yasuhiro; Kitada, Masaaki; Satoh, Fumitoshi; Maekawa, Takashi; Morimoto, Ryo; Yamazaki, Yuto; Ise, Kazue; Gomez-Sanchez, Celso E.; Ito, Sadayoshi; Arai, Yoichi; Dezawa, Mari; Sasano, Hironobu
• Is Part Of: Molecular and cellular endocrinology. Volume 422(2016)
• Page Start: 57
• Page End: 63
• Publication Date: 2016-02-15
• Subjects: Aldosterone-producing adenoma -- CYP11B1 -- CYP11B2 -- CYP17 -- Double-immunostaining -- Triple-immunostaining; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2015.11.014
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Introduction: Cytochrome P450 11B2 (CYP11B2) plays a pivotal role in aldosterone synthesis, while cytochrome P450 11B1 (CYP11B1) and cytochrome P450 17A1 (CYP17) are involved in cortisol synthesis in normal human adrenal glands. However, their detailed distribution in aldosterone-producing adenoma (APA) remains incompletely settled. Materials and methods: We examined the status of CYP11B1/CYP11B2 and CYP11B2/CYP17A1 expressions in 27 APA (double staining) cases and 21 APA (triple staining) cases by using immunofluorescence staining and semi-quantitative evaluation. Results: Tumor cells co-expressing CYP11B1/B2 (hybrid cell type A), CYP11B2/17 (hybrid cell type B), CYP11B1/17 (hybrid cell type C), and CYP11B1/B2/17 (triple-positive cell) were identified. The area and cell number of these cells were relatively small, but the size of individual hybrid cells were different between three hybrid cell types (A/B/C) and triple-positive cells. Conclusion: The presence of hybrid cells indicated the marked intratumoral heterogeneity of steroidogenesis in APAs, particularly in those producing glucocorticoids and mineralocorticoids. Highlights: Tumor cells co-expressing CYP11B1/B2 (hybrid cell type A) were identified in APA. Tumor cells co-expressing CYP11B2/17 (hybrid cell type B) were present in APA. Tumor cells co-expressing CYP11B1/17 (hybrid cell type C) were detected in APA. Tumor cells co-expressing CYP11B1/B2/17 (triple-positive cells) were identified in APA.