Short-term exposure of arsenite disrupted thyroid endocrine system and altered gene transcription in the HPT axis in zebrafish. (October 2015)

• Record Type: Journal Article
• Authors: Sun, Hong-Jie; Li, Hong-Bo; Xiang, Ping; Zhang, Xiaowei; Ma, Lena Q.
• Is Part Of: Environmental pollution. Volume 205(2015)
• Page Start: 145
• Page End: 152
• Publication Date: 2015-10
• Subjects: Arsenite -- Oxidative stress -- Zebrafish -- Thyroid endocrine -- Gene transcription -- Hypothalamic–pituitary–thyroid axis; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2015.05.037
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Arsenic (As) pollution in aquatic environment may adversely impact fish health by disrupting their thyroid hormone homeostasis. In this study, we explored the effect of short-term exposure of arsenite (AsIII) on thyroid endocrine system in zebrafish. We measured As concentrations, As speciation, and thyroid hormone thyroxine levels in whole zebrafish, oxidative stress (H2 O2 ) and damage (MDA) in the liver, and gene transcription in hypothalamic–pituitary–thyroid (HPT) axis in the brain and liver tissues of zebrafish after exposing to different AsIII concentrations for 48 h. Result indicated that exposure to AsIII increased inorganic As in zebrafish to 0.46–0.72 mg kg −1, induced oxidative stress with H2 O2 being increased by 1.4–2.5 times and caused oxidative damage with MDA being augmented by 1.6 times. AsIII exposure increased thyroxine levels by 1.3–1.4 times and modulated gene transcription in HPT axis. Our study showed AsIII caused oxidative damage, affected thyroid endocrine system and altered gene transcription in HPT axis in zebrafish. Graphical abstract: Highlights: 48 h-LC50 value of arsenite (AsIII) was 42 mg L −1 for zebrafish. AsIII exposure elevated oxidative stress and caused oxidative damage in zebrafish. AsIII exposure increased the content of thyroid hormone thyroxine. AsIII exposure altered gene transcription in the HPT axis in zebrafish. Abstract : Short-term exposure of arsenite caused oxidative stress, disrupted thyroid endocrine system and altered gene transcription in the HPT axis in Zebrafish.