Integration of Platinum Nanoparticles with a Volumetric Bar‐Chart Chip for Biomarker Assays1. (17th July 2014)

• Record Type: Journal Article
• Authors: Song, Yujun; Xia, Xuefeng; Wu, Xifeng; Wang, Ping; Qin, Lidong
• Is Part Of: Angewandte Chemie. Volume 126:Number 46(2014)
• Page Start: 12659
• Page End: 12663
• Publication Date: 2014-07-17
• Subjects: Biomarker‐Assays -- Immunassays -- Katalytische Aktivität -- Mikrofluidik -- Platin‐Nanopartikel; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201404349
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Platinum nanoparticles (PtNPs) efficiently catalyze the transformation of H2 O2 into oxygen gas. However, owing to the lack of an efficient approach or device that can measure the produced oxygen gas, the catalytic reaction has never been used for diagnostic applications. Microfluidics technology provides a platform that meets these requirements. The volumetric bar‐chart chip (V‐Chip) volumetrically measures the production of oxygen gas by PtNPs and can be integrated with ELISA technology to provide visible and quantitative readouts without expensive instrumentation or complicated data processing. Herein we show that PtNPs outperform catalase with respect to stability at high H2 O2 concentrations or temperatures or in long‐term reactions, and are resistant to most catalase inhibitors. We also show that the catalase‐like activity of PtNPs can be used in combination with the V‐Chip to sensitively and specifically detect cancer biomarkers both in serum and on the cell surface.