Stable Small Quantum Dots for Synaptic Receptor Tracking on Live Neurons1. (26th September 2014)

• Record Type: Journal Article
• Authors: Cai, En; Ge, Pinghua; Lee, Sang Hak; Jeyifous, Okunola; Wang, Yong; Liu, Yanxin; Wilson, Katie M.; Lim, Sung Jun; Baird, Michelle A.; Stone, John E.; Lee, Kwan Young; Davidson, Michael W.; Chung, Hee Jung; Schulten, Klaus; Smith, Andrew M.; Green, William N.; Selvin, Paul R.
• Is Part Of: Angewandte Chemie. Volume 126:Number 46(2014)
• Page Start: 12692
• Page End: 12696
• Publication Date: 2014-09-26
• Subjects: AMPA‐Rezeptoren -- Einzelmoleküluntersuchungen -- Fluoreszenzsonden -- Quantenpunkte; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201405735
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super‐resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.