Role of Periostin in Early Brain Injury After Subarachnoid Hemorrhage in Mice. Issue 4 (April 2017)

• Record Type: Journal Article
• Authors: Liu, Lei; Kawakita, Fumihiro; Fujimoto, Masashi; Nakano, Fumi; Imanaka-Yoshida, Kyoko; Yoshida, Toshimichi; Suzuki, Hidenori
• Is Part Of: Stroke. Volume 48:Issue 4(2017)
• Publication Date: 2017-04
• Subjects: blood–brain barrier -- early brain injury -- periostin -- subarachnoid hemorrhage -- tenascin-C; Cerebrovascular disease -- Periodicals; Cerebral circulation -- Periodicals; 616.81
• DOI: 10.1161/STROKEAHA.117.016629
• Languages: English
• ISSNs: 0039-2499

Abstract

Abstract : Background and Purpose—: A matricellular protein tenascin-C is implicated in early brain injury after experimental subarachnoid hemorrhage (SAH). This study first evaluated the role of another matricellular protein periostin and the relationships with tenascin-C in post-SAH early brain injury. Methods—: Wild-type (n=226) and tenascin-C knockout (n=9) C57BL/6 male adult mice underwent sham or filament perforation SAH modeling. Vehicle, anti-periostin antibody, or recombinant periostin was randomly administrated by an intracerebroventricular injection at 30 minutes post-modeling. Neuroscores, SAH grading, brain water content, immunostaining, and Western blotting were blindly evaluated at 24 to 48 hours post-SAH. Results—: Periostin was induced in brain capillary endothelial cells and neurons at 24 hours post-SAH. Anti-periostin antibody improved post-SAH neurobehavior, brain edema, and blood–brain barrier disruption associated with downregulation of tenascin-C, inactivation of p38, extracellular signal-related kinase 1/2 and matrix metalloproteinase-9, and subsequent preservation of zona occludens-1. Recombinant periostin aggravated post-SAH brain edema and tenascin-C induction. Tenascin-C knockout prevented post-SAH neurobehavioral impairments and periostin induction. Conclusions—: Periostin may cause post-SAH early brain injury through activating downstream signaling pathways and interacting with tenascin-C, providing a novel approach for the treatment of early brain injury. Abstract : Supplemental Digital Content is available in the text.