Endogenously synthesized n-3 polyunsaturated fatty acids in fat-1 transgenic mice suppress B16F10 melanoma lung metastasis by impairing mesenchymal to epithelial transition. (December 2016)

• Record Type: Journal Article
• Authors: Zhu, Pan; Zhang, Yuan-Ming; Yin, Xuan; Zhang, Xiao-Hong; Wang, Feng; Zhang, Jin-Jie; Yan, Wang; Xi, Yang; Wan, Jian-Bo; Kang, Jing-Xuan; Zou, Zu-Quan; Bu, Shi-Zhong
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 483
• Page End: 490
• Publication Date: 2016-12
• Subjects: Fat-1 -- n-3 polyunsaturated fatty acids -- Mesenchymal to epithelial transition -- Lipid mediators -- IL-1β -- Melanoma; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.10.006
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Endogenous n-3 polyunsaturated fatty acids (PUFA) inhibited lung metastases of B16F10 melanoma cells in fat-1 transgenic mice. The anti-inflammatory status of n-3 PUFAs contributes to lung metastasis inhibition of melanoma cells by impairing MET. Abstract: Malignant melanoma is an aggressive cancer of neuroectodermal origin. The mechanisms of n-3 polyunsaturated fatty acids (PUFAs) against melanoma metastasis are not clear. Here fat-1 and wild type (WT) mice were injected with B16F10 melanoma cells via the tail vein to establish lung metastasis model. Endogenous n-3 PUFAs were associated with a reduction in grossly visible pulmonary metastases and outgrowth through inhibition of mesenchymal to epithelial transition (MET), which was characterized by decreased expression of epithelial markers E-cadherin and ZO-1, and increased expression of mesenchymal marker vimentin in tumour tissues from fat-1 mice compared with WT controls. In addition, n-3 PUFA-mediated inhibition of MET may have been associated, in part, with the: (i) formation of n-3 PUFA-derived lipid mediators and (ii) decreased expression of mature IL-1β and p-NF-κB, and enhanced expression of superoxide dismutase-1. These results suggest that n-3 PUFAs exert their antitumourigenic activities, in part, via their anti-inflammatory properties.