Development of reference material with assigned value for human T‐cell leukemia virus type 1 quantitative PCR in Japan. (21st October 2018)

• Record Type: Journal Article
• Authors: Kuramitsu, Madoka; Okuma, Kazu; Nakashima, Makoto; Sato, Tomoo; Sasaki, Daisuke; Hasegawa, Hiroo; Umeki, Kazumi; Kubota, Ryuji; Sasada, Keiko; Sobata, Rieko; Matsumoto, Chieko; Kaneko, Noriaki; Tezuka, Kenta; Matsuoka, Sahoko; Utsunomiya, Atae; Koh, Ki‐Ryang; Ogata, Masao; Ishitsuka, Kenji; Taki, Mai; Nosaka, Kisato; Uchimaru, Kaoru; Iwanaga, Masako; Sagara, Yasuko; Yamano, Yoshihisa; Okayama, Akihiko; Miura, Kiyonori; Satake, Masahiro; Saito, Shigeru; Watanabe, Toshiki; Hamaguchi, Isao
• Is Part Of: Microbiology and immunology. Volume 62:Number 10(2018)
• Page Start: 673
• Page End: 676
• Publication Date: 2018-10-21
• Subjects: human T‐cell leukemia virus type 1 -- proviral load -- quantitative PCR -- standard; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12644
• Languages: English
• ISSNs: 0385-5600

Abstract

ABSTRACT: Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.