Membrane Protein Sorting: Proteoliposome Engineering with Cell‐Free Membrane Protein Synthesis: Control of Membrane Protein Sorting into Liposomes by Chaperoning Systems (Adv. Sci. 10/2018). Issue 10 (17th October 2018)

• Record Type: Journal Article
• Authors: Ando, Mitsuru; Schikula, Shun; Sasaki, Yoshihiro; Akiyoshi, Kazunari
• Is Part Of: Advanced science. Volume 5:Issue 10(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-17
• Subjects: cell‐free protein synthesis -- hexahistidine‐fused membrane proteins -- nickel‐chelating liposomes -- polyhistidine/nickel‐chelate affinity -- proteoliposomes; Science -- Periodicals; 505
• DOI: 10.1002/advs.201870062
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract : Cell‐free membrane protein (MP) synthesis in the presence of liposomes is a spotlighting technique to prepare MP‐reconstituted liposomes. In article number1800524, Kazunari Akiyoshi and co‐workers develop an artificial MP sorting system based on polyhistidine/nickel‐chelate affinity. This system enables efficient reconstitution of MPs into the liposomal membrane to be achieved, and supplementations of natural molecular chaperone can control the orientation of MPs.