Ratiometric red-emission fluorescence detection of Al3+ in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission. Issue 21 (3rd October 2018)

• Record Type: Journal Article
• Authors: Neupane, Lok Nath; Mehta, Pramod Kumar; Oh, Semin; Park, See-Hyoung; Lee, Keun-Hyeung
• Is Part Of: Analyst. Volume 143:Issue 21(2018)
• Page Start: 5285
• Page End: 5294
• Publication Date: 2018-10-03
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01221k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel peptidyl probe using aggregation induced emission was proposed for the selective ratiometric detection of Al 3+ ions in pure aqueous solutions and live cells. Abstract : The development of a fluorescence method for the selective ratiometric detection of Al 3+ ions in pure aqueous solutions and live cells is still a significant challenge. In the present study, we synthesized a new type of fluorescent probe using an Al 3+ -triggered self-assembly based on the dipeptide receptor and an aggregation-induced emission fluorophore. The fluorescent probe (1 ) bearing cyanostilbene with excitation by visible light detected Al 3+ ions sensitively in pure aqueous buffered solution by ratiometric red-emission at 600 nm.1 provided a highly selective ratiometric detection of Al 3+ among 16 metal ions in aqueous solution.1 exhibited sensitive ratiometric response to Al 3+ in aqueous buffered solutions at pH ranging from 5 to 7.4. The detection limit (145 nM, R 2 = 0.999) for Al 3+ ions in pure aqueous solution was much lower than the maximum allowable level of Al 3+ in drinking water demanded by the Environmental Protection Agency (EPA). The probe provided an efficient approach to detect low concentrations of Al 3+ in ground water, tap water, and live cells by ratiometric red-emissions at 600 nm. The binding study using dynamic light scattering, NMR, IR, and TEM revealed that the complex between1 and Al 3+ self-assembled to form nanoparticles, resulting in the enhancement of the emission at 600 nm and a concomitant decrease in the emission at 535 nm.