Proteomic analysis of 2-monochloropropanediol (2-MCPD) and 2-MCPD dipalmitate toxicity in rat kidney and liver in a 28-days study. (November 2018)

• Record Type: Journal Article
• Authors: Frenzel, Falko; Oberemm, Axel; Braeuning, Albert; Lampen, Alfonso
• Is Part Of: Food and chemical toxicology. Volume 121(2018)
• Page Start: 1
• Page End: 10
• Publication Date: 2018-11
• Subjects: Food processing contaminants -- Heat-induced -- Chloropropanols -- 3-MCPD -- Toxicoproteomics -- Alpha-chlorohydrin; MCPD monochloropropanediol -- EFSA European Food Safety Authority -- IPA ingenuity pathway analysis -- MALDI matrix-assisted laser desorption/ionization -- NFE2L2 nuclear factor (erythroid-derived 2)-like 2; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.08.013
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: 2-monochloropropanediol (2-MCPD), 3-monochloropropanediol (3-MCPD) and their fatty acid esters have recently been identified as heat-induced contaminants in fat- and salt-containing foodstuff. Toxicity of 3-MCPD has been studied previously in some detail. Disturbance of glycolysis and cellular redox functions appear to be involved in 3-MCPD toxicity. By contrast, only very few toxicological data are available for 2-MCPD or 2-MCPD esters, especially at the molecular level. This study was therefore aimed to provide a comprehensive overview of proteomic alterations induced in rat kidney and liver by 2-MCPD and 2-MCPD dipalmitate, a representative 2-MCPD fatty acid ester. Sub-toxic doses of 10 mg/kg body weight 2-MCPD, or equimolar doses of 2-MCPD dipalmitate were applied in a 28-day in vivo gavage oral toxicity study in male rats. Two-dimensional gel electrophoresis and mass-spectrometric protein identification using material from 5 animals per treatment group were employed together with bioinformatic data mining to obtain information about the molecular basis of the observed proteomic alterations. Obtained data indicate toxic consequences of 2-MCPD exposure in the kidney and provide evidence that 2-MCPD exerts its cellular effects in rat kidney by mechanisms different from 3-MCPD. Highlights: Global 2-MCPD effects in rat kidney were analyzed using a proteomics approach. Global 2-MCPD effects in rat liver were analyzed using a proteomics approach. Comparative proteomic analysis of 2-MCPD and 2-MCPD dipalmitate in vivo. Comparative analysis of proteomic and transcriptomic data.