Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay. (December 2017)

• Record Type: Journal Article
• Authors: Shetty, Vinaya; Shetty, NJ; Ananthanarayana, SR; Jha, SK; Chaubey, RC
• Is Part Of: Toxicology and industrial health. Volume 33:Number 12(2017)
• Page Start: 930
• Page End: 937
• Publication Date: 2017-12
• Subjects: Gamma radiation -- DNA damage -- comet assay -- genotoxicity; Toxicology, Experimental -- Periodicals; Industrial toxicology -- Periodicals; 615.9
• DOI: 10.1177/0748233717733599
• Languages: English
• ISSNs: 0748-2337

Abstract

The study was undertaken to evaluate gamma radiation-induced DNA damage in Aedes aegypti . The comet assay was employed to demonstrate the extent of DNA damage produced in adult male A. aegypti exposed to seven different doses of gamma radiation, ranging from 1 Gy to 50 Gy. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received, except in those treated with 1 Gy. The highest amount of DNA damage was noticed at 1 h postirradiation, which decreased gradually with time, that is, at 3, 6 and 12 h postirradiation. This may indicate repair of the damaged DNA and/or loss of heavily damaged cells as the postirradiation time increased. The comet assay serves as a sensitive and rapid technique to detect gamma radiation-induced DNA damage in A. aegypti . This could be used as a potential biomarker for environmental risk assessment.