Validation of an equation predicting highly unsaturated fatty acid (HUFA) compositions of human blood fractions from dietary intakes of both HUFAs and their precursors. (September 2018)

• Record Type: Journal Article
• Authors: Strandjord, Sarah E.; Lands, Bill; Hibbeln, Joseph R.
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 136(2018)
• Page Start: 171
• Page End: 176
• Publication Date: 2018-09
• Subjects: AA arachidonic acid -- ALA alpha-linolenic acid -- DHA docosahexaneoic acid -- GLA gamma-linolenic acid -- en% percent of energy -- EPA eicosapentaenoic acid -- %EPA+DHA in RBC percentage of EPA and DHA in total fatty acids of red blood cell -- HUFA highly unsaturated fatty acids -- LA linoleic acid -- NR not reported -- n-3 omega-3 -- n-6 omega-6 -- %n-6 in HUFA the percentage of omega-6 highly unsaturated fatty acids in total tissue HUFA -- P-PL plasma phospholipids -- P-TL plasma total lipids -- PUFA polyunsaturated fatty acids -- RBC red blood cell -- RBC-PL red blood cell phospholipids -- S-PL serum phospholipids -- SDA stearidonic acid -- S-TL serum total lipids; Blood lipids -- Highly unsaturated fatty acids (HUFA) -- HUFA balance -- Omega-3 -- Omega-6; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2017.03.005
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: Proportions of omega-3 (n-3) and omega-6 (n-6) in 20- and 22-carbon highly unsaturated fatty acids with 3 or more double bonds (HUFA) accumulated in tissue HUFA (e.g., the %n-6 in HUFA) are biomarkers reflecting intakes of n-6 and n-3 fatty acids. An empirical equation, referred to here as the Lands' Equation, was developed previously to use dietary intakes of n-6 and n-3 HUFA and their 18-carbon precursors to estimate the %n-6 in HUFA of humans. From the PubMed database, we identified clinical trials reporting (a) dietary intake of at least linoleic acid (18:2n-6) and alpha-linolenic acid (18:3n-3), and (b) the amounts of at least arachidonic acid (20:4n-6), eicosapentaenoic acid (20:5n-3), and docosahexaenoic acid (22:6n-3) in lipids of plasma, serum, or red blood cell. Linear regression analyses comparing reported and predicted %n-6 in HUFA gave a correlation coefficient of 0.73 ( p <0.000000) for 34 studies with 92 subject groups. These results indicate that circulating HUFA compositions can be reliably estimated from dietary intake data that not only includes n-3 and n-6 HUFA consumption, but also includes consumption of 18 carbon n-3 and n-6 precursor fatty acids. Highlights: Omega-3/omega-6 highly unsaturated fatty acids (HUFA) are disease risk biomarkers. Precursor dietary fats are not typically included in estimating n-3 and n-6 HUFAs. Reliable HUFA biomarkers estimates require both dietary precursor fats and HUFAs.