Distinguishing nuclei-specific benzo[a]pyrene-induced effects from whole-cell alterations in MCF-7 cells using Fourier-transform infrared spectroscopy. (1st September 2015)

• Record Type: Journal Article
• Authors: Obinaju, Blessing E.; Fullwood, Nigel J.; Martin, Francis L.
• Is Part Of: Toxicology. Volume 335(2015)
• Page Start: 27
• Page End: 34
• Publication Date: 2015-09-01
• Subjects: ATR-FTIR spectroscopy -- Benzo[a]pyrene -- DNA damage -- MCF-7 cells -- Nucleus isolation -- Polycyclic aromatic hydrocarbon; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2015.07.001
• Languages: English
• ISSNs: 0300-483X

Abstract

Abstract: Exposure to chemicals such as benzo[ a ]pyrene (B[ a ]P) can generate intracellular toxic mechanisms. Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes. This study set out to examine whether B[ a ]P-induced whole cell alterations could be distinguished from effects on nuclei of exposed cells. Using attenuated total reflection FTIR (ATR–FTIR) spectroscopy, alterations in nuclei isolated from B[ a ]P-treated MCF-7 cells concentrated either in G0 /G1 - or S-phase were observed. B[ a ]P-induced effects in whole-cells included alterations to lipids, DNA and protein spectral regions. Absorbance areas for protein and DNA/RNA regions in B[ a ]P-treated whole cells differed significantly ( P < 0.0001) from vehicle controls and these observations correlated with alterations noted in isolated nuclei. Our findings provide evidence that FTIR spectroscopy has the ability to identify specific chemical-induced alterations.