Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability. Issue 45 (29th October 2018)

• Record Type: Journal Article
• Authors: Avendaño, Catalina; Jenkins, Mark; Méndez-Callejas, Gina; Oviedo, Jairo; Guzmán, Fanny; Patarroyo, Manuel A.; Sánchez-Acedo, Caridad; Quílez, Joaquín
• Is Part Of: Vaccine. Volume 36:Issue 45(2018)
• Page Start: 6703
• Page End: 6710
• Publication Date: 2018-10-29
• Subjects: Cryptosporidium -- Cryptosporidiosis -- Peptide -- Vaccine -- CP15 -- CSL; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2018.09.044
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: Cryptosporidium spp. CP15 and CSL peptides stimulate antibody production. Antibodies induced by CP15-1 and CP15-3 peptides neutralise parasite entry in vitro. CP15 plays an important role in sporozoite entry to enterocytes. A multiple-epitope vaccine is required for complete entry blocking. Abstract: Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.