Regional surveillance of parasitic infections in wild Northern Bobwhite Quail (Colinus virginianus) utilizing a mobile research laboratory platform. (13th June 2018) - Blanchard, Kendall R.
Abstract: Northern bobwhite quail ( Colinus virginianus ), a popular gamebird among hunters, have been declining over recent decades in the Rolling Plains ecoregion. Investigations in the past few years have revealed a high prevalence of eyeworms ( Oxyspirura petrowi ) and caecal worms ( Aulonocephalus pennula ) in this ecoregion, prompting a need to better understand their host–parasite interaction and other factors that influence infection. In this study, the efficiency of a mobile laboratory was tested by deploying it to three field sites in the Rolling Plains between July and August of 2017 and collecting cloacal swabs from bobwhites. The DNA was extracted from swabs for quantitative PCR and was run in the mobile and reference laboratory to specifically detect A. pennula and O. petrowi infection. When compared with the Wildlife Toxicology's reference laboratory, the mobile laboratory had a 97 and 99% agreement for A. pennula and O. petrowi, respectively. There were no significant differences in infection levels between field sites. Due to its efficiency, it is proposed that the mobile laboratory would be an effective way to monitor infection levels, in addition to factors that may affect infection such as climate, diapause, and intermediate host populations.