Whole genome amplification and exome sequencing of archived schistosome miracidia. Issue 13 (28th May 2018)

• Record Type: Journal Article
• Authors: Le Clec'h, Winka; Chevalier, Frédéric D.; McDew-White, Marina; Allan, Fiona; Webster, Bonnie L.; Gouvras, Anouk N.; Kinunghi, Safari; Tchuem Tchuenté, Louis-Albert; Garba, Amadou; Mohammed, Khalfan A.; Ame, Shaali M.; Webster, Joanne P.; Rollinson, David; Emery, Aidan M.; Anderson, Timothy J. C.
• Editors: Stothard, J. Russell; Webster, Bonnie L.
• Is Part Of: Parasitology. Volume 145:Issue 13(2018)
• Page Start: 1739
• Page End: 1747
• Publication Date: 2018-05-28
• Subjects: Exome sequencing, -- FTA cards, -- miracidia, -- quantitative PCR, -- Schistosoma, -- whole genome amplification; Parasitology -- Periodicals; Electronic journals; 616.96
• DOI: 10.1017/S0031182018000811
• Languages: English
• ISSNs: 0031-1820

Abstract

Abstract: Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through theS chistosomiasisC ollectiona t theN atural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and S chistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.