Cigarette smoke condensate alters Saccharomyces cerevisiae efflux transporter mRNA and activity and increases caffeine toxicity. (1st November 2018)

• Record Type: Journal Article
• Authors: Sayyed, Katia; Le Vée, Marc; Chamieh, Hala; Fardel, Olivier; Abdel-Razzak, Ziad
• Is Part Of: Toxicology. Volume 409(2018)
• Page Start: 129
• Page End: 136
• Publication Date: 2018-11-01
• Subjects: ABC transporter -- Pdr5 -- Snq2 -- Cigarette smoke -- Caffeine -- Saccharomyces cerevisiae; ABC ATP-binding cassette -- AαC 2-amino-9H-pyrido[23-b]indole -- 4- ABP 4-aminobiphenyl -- BaP benzo(a)pyrene -- BLAST Basic local alignment search tool -- BCRP breast cancer resistant protein -- CS cigarette smoke -- CSE cigarette smoke extract -- CSC cigarette smoke condensate -- DMSO dimethyl sulfoxide -- GST glutathione-S- transferase -- IC50 half-maximal inhibitory concentration -- HEK-293 human embryonic kidney cells 293 -- MFS major facilitator superfamily -- MDR multi-drug resistance -- MRP multidrug resistance protein -- OD optic density -- PhIP 2-amino-1-methyl-6-phenylimidazo45-b)pyridine -- PDRE Pdr1p/Pdr3p response element -- P-gp P-glycoprotein -- PBS phosphate-buffered saline -- PDR pleiotropic drug resistance -- PAH polycyclic aromatic hydrocarbons -- qPCR quantitative polymerase chain reaction -- Rh123 rhodamine 123 -- RT reverse-transcription -- S. cerevisiae Saccharomyces cerevisiae -- TPO polyamine transporter -- YPD yeast peptone dextrose; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2018.08.005
• Languages: English
• ISSNs: 0300-483X

Abstract

Abstract: In animals, cigarette smoke may alter pharmacokinetics by altering activity and expression of ABC drug transporters. We previously demonstrated that cigarette smoke condensate (CSC) impairs activity and expression of several hepatic ABC drug transporters which mediate toxicant efflux. However, CSC effects on efflux transporters are still unknown in Saccharomyces cerevisiae which resists diverse chemical stresses, by inducing pleiotropic drug resistance (PDR) genes among others. The yeast ABC transporters are functionally and structurally homologous to the mammalian ones. In this study, Saccharomyces cerevisiae exposure to CSC for 15 min caused a dose-dependent inhibition of rhodamine 123 efflux, whereas a longer exposure (3 h) induced mRNA expression of the ABC PDR efflux pumps Pdr5, Snq2, Pdr 10 and Pdr15, and of Tpo1, a member of the major facilitator superfamily (MFS). CSC also increased toxicity of caffeine, which is handled by two PDR transporters, Pdr5 and Snq2. Taken together, these data demonstrated that yeast efflux transporters are targets of cigarette smoke chemicals, and that Saccharomyces cerevisiae may cope with CSC-induced stress, including the initial efflux inhibition, by induction of the mRNA of several plasma membrane PDR and MFS efflux transporters. Saccharomyces cerevisiae is therefore a valid model to investigate pollutant effects on ABC and MFS transporters.