A ratiometric fluorescent probe for the detection of peroxynitrite with simple synthesis and large emission shift and its application in cells image. (February 2019)

• Record Type: Journal Article
• Authors: Zhou, Dong-Ye; Ou-Yang, Juan; Li, Yongfei; Jiang, Wen-Li; Tian, Yang; Yi, Zheng-Ming; Li, Chun-Yan
• Is Part Of: Dyes and pigments. Volume 161(2019)
• Page Start: 288
• Page End: 295
• Publication Date: 2019-02
• Subjects: Fluorescent probe -- Peroxynitrite -- Simple synthesis -- Large emission shift -- Cells image; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2018.09.059
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Peroxynitrite (ONOO − ) is an important kind of reactive oxygen species (ROS), played an essential role in physiology and pathology. To date, various fluorescent probes for ONOO − have been constructed successfully, but these probes exhibit turn-on fluorescence signal in single channel. While, ratiometric fluorescent probes, especially those probes with large emission shift, are suitable for bioimage in two channels. Herein, a hemicyanine-based fluorescent probe named Cy-NH2 was designed and synthesized for ratiometric detection of ONOO − . The characteristics of Cy-NH2 are as follows: (1) The probe possesses a simple structure, which can be obtained by easy synthetic steps; (2) The ratiometric fluorescent probe owns large emission shift (248 nm) due to the disruption of π-conjugation system caused by strong oxidizing ability of ONOO − ; (3) The probe exhibits high sensitivity to ONOO − with 1728-fold enhancement of fluorescence intensity ratio and superior selectivity for ONOO − over various potentially interfering biological analytes; (4) The ratiometric fluorescent probe is applied for the image detection of exogenous and endogenous ONOO − in living cells successfully. Graphical abstract: Highlights: A ratiometric fluorescent probe (Cy-NH2 ) is developed for detecting ONOO − . The probe possesses a simple structure and can be obtained by easy synthetic steps. The probe owns large emission shift due to the disruption of conjugation system. The ratiometric fluorescent probe shows high sensitivity and selectivity to ONOO − . The probe has been applied for the detection of ONOO − in living cells successfully.