Sequentially self-assembled polysaccharide-based nanocomplexes for combined chemotherapy and photodynamic therapy of breast cancer. (1st January 2019)

• Record Type: Journal Article
• Authors: Wang, Yaping; Yang, Ming; Qian, Junmin; Xu, Weijun; Wang, Jinlei; Hou, Guanghui; Ji, Lijie; Suo, Aili
• Is Part Of: Carbohydrate polymers. Volume 203(2019)
• Page Start: 203
• Page End: 213
• Publication Date: 2019-01-01
• Subjects: Hyaluronic acid -- Hydroxyethyl chitosan -- Nanocomplexes -- pH-Responsivity -- Chemo-Photodynamic dual therapy -- Breast cancer; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.09.035
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Multifunctional hyaluronic acid/hydroxyethyl chitosan nanocomplexes were prepared. DOX and ALA were loaded into polysaccharide nanocomplexes via Schiff base linkage. Nanocomplexes displayed pH-responsive surface charge reversal and drug release. Active targeting enhanced additive effect of chemotherapy and photodynamic therapy. Abstract: Combination of chemotherapy and photodynamic therapy has emerged as a promising anticancer strategy. Polysaccharide-based nanoparticles are being intensively explored as drug carriers for different forms of combination therapy. In this study, novel multifunctional polysaccharide-based nanocomplexes were prepared from aldehyde-functionalized hyaluronic acid and hydroxyethyl chitosan via sequential self-assembly method. Stable nanocomplexes were obtained through both Schiff's base bond and electrostatic interactions. Chemotherapeutics doxorubicin and pro-photosensitizer 5-aminolevulinic acid were chemically conjugated onto the nanocomplexes via Schiff base linkage. Anti-HER2 antibody as targeting moiety was decorated onto the surface of nanocomplexes. The obtained near-spherical shaped nanocomplexes had an average size of 140 nm and a zeta potential of −24.6 mV, and displayed pH-responsive surface charge reversal and drug release. Active targeting strategy significantly enhanced the cellular uptake of nanocomplexes and combined anticancer efficiency of chemo-photodynamic dual therapy in breast cancer MCF-7 cells. These results suggested that the nanocomplexes had great potential for targeted combination therapy of breast cancer.