Biochar does not attenuate triclosan's impact on soil bacterial communities. (December 2018)

• Record Type: Journal Article
• Authors: Phandanouvong-Lozano, Vienvilay; Sun, Wen; Sanders, Jennie M.; Hay, Anthony G.
• Is Part Of: Chemosphere. Volume 213(2018)
• Page Start: 215
• Page End: 225
• Publication Date: 2018-12
• Subjects: Biodegradation -- Xenobiotic -- Biosolid -- Pollutant -- Metagenome; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.08.132
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Triclosan, a broad-spectrum antimicrobial, has been widely used in pharmaceutical and personal care products. It undergoes limited degradation during wastewater treatment and is present in biosolids, most of which are land applied in the United States. This study assessed the impact of triclosan (0–100 mg kg −1 ) with and without biochar on soil bacterial communities. Very little 14 C-triclosan was mineralized to 14 CO2 (<7%) over the course of the study (42 days). While biochar (1%) significantly lowered mineralization of triclosan, analysis of 16S rRNA gene sequences revealed that biochar impacted very few OTUs and did not alter the overall structure of the community. Triclosan, on the other hand, significantly affected bacterial diversity and community structure (alpha diversity, ANOVA, p < 0.001; beta diversity, AMOVA, p < 0.01). Dirichlet multinomial mixtures (DMM) modeling and complete linkage clustering (CLC) revealed a dose-dependent impact of triclosan. Non-Parametric Metastats (NPM) analysis showed that 150 of 734 OTUs from seven main phyla were significantly impacted by triclosan (adjusted p < 0.05). Genera harboring opportunistic pathogens such as Flavobacterium were enriched in the presence of triclosan, as was Stenotrophomonas . The latter has previously been implicated in triclosan degradation via stable isotope probing. Surprisingly, Sphingomonads, which include well-characterized triclosan degraders were negatively impacted by even low doses of triclosan. Analyses of published genomes showed that triclosan resistance determinants were rare in Sphingomonads which may explain why they were negatively impacted by triclosan in our soil. Highlights: Biochar reduced triclosan mineralization. Triclosan was the main driver of bacterial community differences. Taxa harboring opportunistic pathogens were enriched in soil receiving triclosan. Predicted gene functions: resistance at 10 mg kg −1, degradation at 100 mg kg −1 . Relatives of known triclosan degraders were negatively impacted by triclosan.