Correlation of intracellular oxygen and cell metabolism by simultaneous PLIM of phosphorescent TLD1433 and FLIM of NAD(P)H. Issue 10 (9th July 2018)

• Record Type: Journal Article
• Authors: Kalinina, Sviatlana; Breymayer, Jasmin; Reeß, Kirsten; Lilge, Lothar; Mandel, Arkady; Rück, Angelika
• Is Part Of: Journal of biophotonics. Volume 11:Issue 10(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-07-09
• Subjects: autofluorescence -- cell metabolism -- fluorescence lifetime microscopy -- oxygen consumption -- redox state; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201800085
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : During photodynamic therapy (PDT), disruption of cell respiration and metabolic changes could be one of the first events. Photophysical characteristics of the photosensitizer (PS) and its specific redox potential define consumption of molecular oxygen followed by generation of reactive oxygen species. The potential PS TLD1433 is based on transition metal Ru(II) and possess an oxygen‐dependent luminescence. This enables the study of oxygen consumption by PS‐phosphorescence lifetime imaging (PLIM) and simultaneously changes the cellular metabolic state by nicotinamide adenine dinucleotide (NAD(P)H)‐fluorescence lifetime imaging (FLIM). Within this study, localization and cellular function of TLD1433 is investigated in bladder carcinoma cells using time‐resolved and confocal laser scanning microscopy. Simultaneous FLIM/PLIM of NAD(P)H and TLD1433 during PDT correlated oxygen consumption, redox state and cellular energy metabolism. Our investigations aimed to provide a personalized protocol in theranostic PDT procedures and demonstrate the potential use of TLD1433 PDT also under hypoxic conditions, which are otherwise difficult to treat. Abstract : Use of time‐correlated single photon counting techniques (TCSPCs) and multiphoton laser scanning microscopy to provide simultaneous imaging of the fluorescence lifetime imaging (FLIM) of cellular metabolic coenzymes and oxygen‐sensitive phosphorescence lifetime imaging (PLIM) of ruthenium (II) complexes. Here results of applying of TLD1433, a potential photosensitizer are demonstrated. FLIM/PLIM correlates well with PDT‐induced cellular effects induced during prolonged laser‐scanning microcopy.