Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents. Issue 81 (26th September 2018)
- Record Type:
- Journal Article
- Title:
- Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents. Issue 81 (26th September 2018)
- Main Title:
- Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents
- Authors:
- Zou, Zhi
Alibiglou, Hoda
Mate, Diana M.
Davari, Mehdi D.
Jakob, Felix
Schwaneberg, Ulrich - Abstract:
- Abstract : Evolved sortase A variants enable efficient site-specific bioconjugations in organic co-solvents. Abstract : Directed sortase A evolution yielded the variants R159G and D165Q/D186G/K196V with increased resistance (2.2-fold) and catalytic efficiency (6.3-fold) in 45% (v/v) dimethylsulfoxide. Interestingly, D165Q/D186G/K196V also showed an up to 4.7-fold increased activity for the conjugation of hydrophobic peptides/amines in co-solvents. MD simulations revealed that conformational mobilities are important for the gained resistance.
- Is Part Of:
- Chemical communications. Volume 54:Issue 81(2018)
- Journal:
- Chemical communications
- Issue:
- Volume 54:Issue 81(2018)
- Issue Display:
- Volume 54, Issue 81 (2018)
- Year:
- 2018
- Volume:
- 54
- Issue:
- 81
- Issue Sort Value:
- 2018-0054-0081-0000
- Page Start:
- 11467
- Page End:
- 11470
- Publication Date:
- 2018-09-26
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c8cc06017g ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 7944.xml