A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering. Issue 20 (24th September 2018)

• Record Type: Journal Article
• Authors: Zeng, Chen; Hu, Fanghao; Long, Rong; Min, Wei
• Is Part Of: Analyst. Volume 143:Issue 20(2018)
• Page Start: 4844
• Page End: 4848
• Publication Date: 2018-09-24
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00910d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy. Abstract : Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2 S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H2 S level changes in living cells.