Application of non-canonical crosslinking amino acids to study protein–protein interactions in live cells. (October 2018)

• Record Type: Journal Article
• Authors: Coin, Irene
• Is Part Of: Current opinion in chemical biology. Volume 46(2018)
• Page Start: 156
• Page End: 163
• Publication Date: 2018-10
• Subjects: Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2018.07.019
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract : The genetic incorporation of non-canonical amino acids (ncAAs) equipped with photo-crosslinking and chemical crosslinking moieties has found broad application in the study of protein–protein interactions from a unique perspective in live cells. We highlight here applications of photo-activatable ncAAs to map protein interaction surfaces and to capture protein–protein interactions, and we describe recent efforts to efficiently couple photo-crosslinking with mass spectrometric analysis. In addition, we describe recent advances in the development and application of ncAAs for chemical crosslinking, including protein stapling, photo-control of protein conformation, two-dimensional crosslinking, and stabilization of transient and low-affinity protein–protein interactions. We expect that the field will keep growing in the near future and enable the tackling of ambitious biological questions.