Fabrication and evaluation of carbonate apatite‐coated calcium carbonate bone substitutes for bone tissue engineering. (29th August 2018)

• Record Type: Journal Article
• Authors: Fujioka‐Kobayashi, Masako; Tsuru, Kanji; Nagai, Hirokazu; Fujisawa, Kenji; Kudoh, Takaharu; Ohe, Go; Ishikawa, Kunio; Miyamoto, Youji
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 12:Number 10(2018)
• Page Start: 2077
• Page End: 2087
• Publication Date: 2018-08-29
• Subjects: bone regeneration -- bone substitute -- bone tissue engineering -- calcium carbonate -- carbonate apatite -- osteoconductivity; Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.2742
• Languages: English
• ISSNs: 1932-6254

Abstract

Abstract: Carbonate apatite‐coated calcium carbonate (CO3 Ap/CaCO3 ) was fabricated through a dissolution–precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3 Ap shell, along with Ca 2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3, 10% CO3 Ap/CaCO3, 30% CO3 Ap/CaCO3, and CO3 Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca 2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3 Ap. Interestingly, in a rabbit femur defect model, 10% CO3 Ap/CaCO3 induced significantly higher new bone formation and higher material resorption compared with CO3 Ap at 8 weeks. Nevertheless, CO3 Ap showed a superior osteoconductive potential compared with 10% CO3 Ap/CaCO3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO3 Ap/CaCO3 has a positive potential for bone tissue engineering based on well‐controlled calcium release, bone formation, and material resorption.