Intracellular Electrochemical Sensing. Issue 10 (25th July 2018)

• Record Type: Journal Article
• Authors: Ino, Kosuke; Nashimoto, Yuji; Taira, Noriko; Azcon, Javier Ramon; Shiku, Hitoshi
• Is Part Of: Electroanalysis. Volume 30:Issue 10(2018)
• Page Start: 2195
• Page End: 2209
• Publication Date: 2018-07-25
• Subjects: Micro/nanoelectrode -- Analytical electrochemistry -- Intracellular sensing -- Cell analysis; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201800410
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ . Abstract :