Preparation of 68Ga-labelled DOTA-peptides using a manual labelling approach for small-animal PET imaging. (January 2016)

• Record Type: Journal Article
• Authors: Romero, Eduardo; Martínez, Alfonso; Oteo, Marta; García, Angel; Morcillo, Miguel Angel
• Is Part Of: Applied radiation and isotopes. Volume 107(2016:Jan.)
• Page Start: 113
• Page End: 120
• Publication Date: 2016-01
• Subjects: Gallium-68 -- Concentration -- Purification -- NaOH -- Radiolabelling -- DOTA-peptides -- Pheochromocytoma; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2015.10.005
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: 68 Ga-DOTA-peptides are a promising PET radiotracers used in the detection of different tumours types due to their ability for binding specifically receptors overexpressed in these. Furthermore, 68 Ga can be produced by a 68 Ge/ 68 Ga generator on site which is a very good alternative to cyclotron-based PET isotopes. Here, we describe a manual labelling approach for the synthesis of 68 Ga-labelled DOTA-peptides based on concentration and purification of the commercial 68 Ga/ 68 Ga generator eluate using an anion exchange-cartridge. 68 Ga-DOTA-TATE was used to image a pheochromocytoma xenograft mouse model by a microPET/CT scanner. The method described provides satisfactory results, allowing the subsequent 68 Ga use to label DOTA-peptides. The simplicity of the method along with its implementation reduced cost, makes it useful in preclinical PET studies. Highlights: Concentration and purification method using anion exchange resin for 68 Ga synthesis. Manual radiolabelling DOTA-TATE. Small-animal pheochromocytoma tumour PET imaging.