Comparison of steam-alkali-chemical and microwave-alkali pretreatment for enhancing the enzymatic saccharification of oil palm trunk. (December 2016)

• Record Type: Journal Article
• Authors: Lai, Long Wee; Idris, Ani
• Is Part Of: Renewable energy. Volume 99(2016)
• Page Start: 738
• Page End: 746
• Publication Date: 2016-12
• Subjects: Cellulase -- Composition determination -- Crystallinity index -- Lignocellulose -- Morphology analyses -- Structure characterization; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2016.07.059
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: This paper demonstrates two different pretreatment protocols for oil palm trunks (OPT); steam-alkali-chemical (SAC) and microwave-alkali (Mw-A) method. The composition, morphology, structure and crystallinity of OPT before and after pretreatment were analyzed. The effectiveness of the pretreated methods was investigated by performing enzymatic saccharification on the OPT. The physiochemical factors namely: enzyme ratio (cellulase to β-glucosidase), pH, temperature and substrate loading (w/v) on enzymatic saccharification were also investigated. The pre-determined optimal conditions were then used for further enzymatic hydrolysis of raw and pretreated OPT substrates. The results revealed a huge degree of reduction in lignin, up to 89% for SAC treated OPT and at least 15% for Mw-A treated OPT sample as compared to untreated ones. High glucose accumulation (79.4%) was obtained after 72 h saccharification for both pretreated OPT samples. Graphical abstract: Highlights: Steam-alkali-chemical process was used in oil palm trunk pretreatment. Microwave-alkali method was employed. Both methods exhibited significant effects on OPT chemical constituents. The crystallinity structure was disrupted forming more amorphous structure. All pretreated OPT sample gave higher glucose accumulation up to 79.4%.