Adsorption–synergic biodegradation of diesel oil in synthetic seawater by acclimated strains immobilized on multifunctional materials. Issue 1 (15th March 2015)

• Record Type: Journal Article
• Authors: Wang, Xin; Wang, Xuejiang; Liu, Mian; Bu, Yunjie; Zhang, Jing; Chen, Jie; Zhao, Jianfu
• Is Part Of: Marine pollution bulletin. Volume 92:Issue 1/2(2015)
• Page Start: 195
• Page End: 200
• Publication Date: 2015-03-15
• Subjects: Diesel oil -- Adsorption -- Biodegradation -- Bio-carrier -- Immobilization; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2014.12.033
• Languages: English
• ISSNs: 0025-326X

Abstract

Highlights: Three oil degrading bacteria isolated from oil contaminated seawater. The bacteria were immobilized on the hydrophobic bio-carriers and gave an adsorption–synergic biodegradation. The floatability of bio-carriers made them easy to recover. Abstract: Using enrichment culture technique, three isolates marked as ODB-1, ODB-2 and ODB-3, were selected from oil contaminated seawater. 16S rDNA gene sequencing indicated that ODB-1 affiliated with Pseudomonas sp. while ODB-2 and ODB-3 affiliated with Brevundimonas sp. Subsequently, the bacterial cells were immobilized on the surface of expanded graphite (EG), expanded perlite (EP) and bamboo charcoal (BC). Among the three isolates, ODB-1 showed a strong binding to the bio-carriers through extracellular polysaccharides, while ODB-2 and ODB-3 made the adhesion to bio-carrier through direct physical adsorption. The immobilized bacteria exhibited good salinity tolerance compared with the planktonic bacteria. Their total diesel oil removal rates were more than 85% after 6 days' incubation. Adsorption–biodegradation process played an important role in the oil-pollution remediation. EG-bacteria system was treated as a promising remediation method, which achieved nearly 100% removal of diesel oil. Thereinto, over 83% removal of diesel oil owed to biodegradation.