Metabarcoding approach for the ballast water surveillance – An advantageous solution or an awkward challenge?. Issue 1 (15th March 2015)

• Record Type: Journal Article
• Authors: Zaiko, Anastasija; Martinez, Jose L.; Schmidt-Petersen, Julia; Ribicic, Deni; Samuiloviene, Aurelija; Garcia-Vazquez, Eva
• Is Part Of: Marine pollution bulletin. Volume 92:Issue 1/2(2015)
• Page Start: 25
• Page End: 34
• Publication Date: 2015-03-15
• Subjects: Invasive species -- Ballast water management convention -- COI -- RuBisCO -- High-throughput sequencing -- En-route observation; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2015.01.008
• Languages: English
• ISSNs: 0025-326X

Abstract

Highlights: Metabarcoding was compared to the visual survey of ballast water onboard RV. Both approaches revealed similar trend in ballast water community dynamics. A rapid decline in phytoplankton taxa was noticed during the first days of voyage. The metazoans demonstrated evident drop during the second week of observations. Protozoans and water molds dominated the community at the end of the experiment. Abstract: Transfer of organisms with ships' ballast water is recognized as a major pathway of non-indigenous species introduction and addressed in a few recent legislative initiatives. Among other they imply scientific and technical research and monitoring to be conducted in a efficient and reliable way. The recent development of DNA barcoding and metabarcoding technologies opens new opportunities for biodiversity and biosecurity surveillance. In the current study, the performance of metabarcoding approach was assessed in comparison to the conventional (visual) observations, during the en route experimental ballast water survey. Opportunities and limitations of the molecular method were identified from taxonomical datasets rendered by two molecular markers of different degree of universality – the universal cytochrome oxydase sub-unit I gene and a fragment of RuBisCO gene. The cost-efficacy and possible improvements of these methods are discussed for the further successful development and implementation of the approach in ballast water control and NIS surveillance.