Leveraging Reciprocity to Identify and Characterize Unknown Allosteric Sites in Protein Tyrosine Phosphatases. Issue 15 (21st July 2017)

• Record Type: Journal Article
• Authors: Cui, Danica S.; Beaumont, Victor; Ginther, Patrick S.; Lipchock, James M.; Loria, J. Patrick
• Is Part Of: Journal of molecular biology. Volume 429:Issue 15(2017)
• Page Start: 2360
• Page End: 2372
• Publication Date: 2017-07-21
• Subjects: CSP chemical shift perturbation -- PTP protein tyrosine phosphatase -- PTP1B protein tyrosine phosphatase 1B -- VHR Vaccinia H1-related phosphatase -- BB30 benzofuran-based small molecule -- pNPP p-nitrophenylphosphate -- SASA solvent-accessible surface areas; Protein tyrosine phosphatase -- NMR spectroscopy -- Allostery -- NMR -- Chemical Shift; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2017.06.009
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Drug-like molecules targeting allosteric sites in proteins are of great therapeutic interest; however, identification of potential sites is not trivial. A straightforward approach to identify hidden allosteric sites is demonstrated in protein tyrosine phosphatases (PTP) by creation of single alanine mutations in the catalytic acid loop of PTP1B and VHR. This approach relies on the reciprocal interactions between an allosteric site and its coupled orthosteric site. The resulting NMR chemical shift perturbations (CSPs) of each mutant reveal clusters of distal residues affected by acid loop mutation. In PTP1B and VHR, two new allosteric clusters were identified in each enzyme. Mutations in these allosteric clusters altered phosphatase activity with changes in k cat / K M ranging from 30% to nearly 100-fold. This work outlines a simple method for identification of new allosteric sites in PTP, and given the basis of this method in thermodynamics, it is expected to be generally useful in other systems. Graphical Abstract: Highlights: Hidden allosteric networks can be identified by examining the protein-wide 1 H, 15 N CSPs of active site mutations. Frequency analysis of CSP of alanine mutations in the catalytic acid loop identified five clusters of distal residues in PTP1B and two in VHR that are affected by active site perturbations. Mutations in these distal clusters are shown to affect the catalytic efficiency and loop conformations of the phosphatases in study.