Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates. Issue 18 (15th September 2016)

• Record Type: Journal Article
• Authors: Cellier, Marie; James, Arthur L.; Lowe, Jonathan; Orenga, Sylvain; Perry, John D.; Rasul, Ari K.; Stanforth, Stephen P.
• Is Part Of: Bioorganic & medicinal chemistry. Volume 24:Issue 18(2016)
• Page Start: 4066
• Page End: 4074
• Publication Date: 2016-09-15
• Subjects: Fluorogenic enzyme substrates -- l-Alanylaminopeptidase detection -- Microorganisms; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2016.06.051
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifyingl -alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.