Residual antibiofilm effects of various concentrations of double antibiotic paste used during regenerative endodontics after different application times. (October 2016)

• Record Type: Journal Article
• Authors: Jenks, Daniel B.; Ehrlich, Ygal; Spolnik, Kenneth; Gregory, Richard L.; Yassen, Ghaeth H.
• Is Part Of: Archives of oral biology. Volume 70(2016)
• Page Start: 88
• Page End: 93
• Publication Date: 2016-10
• Subjects: Double antibiotic paste -- Calcium hydroxide -- Antibacterial effect -- Enterococcus faecalis -- Bacterial biofilm; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2016.06.006
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: We investigated the residual antibacterial effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks. Dentin pretreatment with 5 mg/mL of DAP or higher demonstrated significant and substantial residual antibacterial effects regardless of the treatment time. Dentin pretreatment for 4 weeks with 5 mg/mL of DAP or higher demonstrated significantly higher residual antibacterial effects in comparison to a 1 week pretreatment with similar concentrations. Dentin pretreatment with calcium hydroxide did not demonstrate any substantial residual antibacterial effect regardless of the treatment time. Abstract: Objective: We investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks. Design: Dentin samples were prepared (n = 120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500 mg/mL), low concentrations of DAP (1, 5 or 50 mg/mL) loaded into a methylcellulose system, calcium hydroxide (Ca(OH)2 ), or placebo paste. After the assigned treatment time, treatment pastes were rinsed off and the samples were kept independently in phosphate buffered saline for 3 weeks. Pretreated dentin samples were then inoculated with Enterococcus faecalis and bacterial biofilms were allowed to grow for an additional 3 weeks. Biofilms were then retrieved from dentin using biofilm disruption assays, diluted, spiral plated, and quantified. Fisher's Exact and Wilcoxon rank sum tests were used for statistical comparisons (α=0.05). Results: Dentin pretreatment for 4 weeks with 5, 50 or 500 mg/mL of DAP demonstrated significantly higher residual antibiofilm effects and complete eradication of E. faecalis biofilms in comparison to a 1 week pretreatment with similar concentrations. However, dentin pretreated with 1 mg/mL of DAP or Ca(OH)2 did not provide a substantial residual antibiofilm effect regardless of the application time. Conclusions: Dentin pretreatment with 5 mg/mL of DAP or higher for 4 weeks induced significantly higher residual antibiofilm effects in comparison to a 1 week pretreatment with the same concentrations.